Dissolution DNP NMR with solvent mixtures: Substrate concentration and radical extraction
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چکیده
منابع مشابه
Dissolution DNP NMR with solvent mixtures: substrate concentration and radical extraction.
Dynamic nuclear polarization (DNP) followed by sudden sample dissolution, is a topic of active investigation owing to the method's unique prospects for the delivery of NMR spectra and images with unprecedented sensitivity. This experiment achieves hyperpolarization by the combined effects of electron-nuclear irradiation and cryogenic operation; the exploitation of these states occurs following ...
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Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Barcelona, Spain. b Servei de Ressonància Magnètica Nuclear, Facultat de Ciències i Biociències, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Catalonia, Spain. ‡ These authors have contributed equally. †Electronic Supplementary Information (ESI) available: [Synthesis and characterization of free radicals 1-7, EPR spe...
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The extraction of Gadolinium (Gd) from perchlorate and nitrate media in 1,2-dichloroethane(C2H4Cl2), carbon tetrachloride (CCl4) and chloroform (CHCl3) by mixtures of 2-thenoyltrifluoroacetone (HTTA) and a crown ether benzo-15-crown-5 (B15C5) is investigated.The composition of the extracted species is determined as Gd(TTA)3.nB15C5 (n=1 in nitratemedium and n=2 in perchlorate medium). The values...
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The free radical 2,2-diphenyl-1-pycrylhydrazyl (DPPH) was tested as a polarising agent for fast dissolution dynamic nuclear polarisation (DNP) NMR spectroscopy. DPPH was found to be reasonably soluble in sulfolane and the optimum concentration for DNP is 20-40 mM depending upon whether short polarisation times or the maximum signal intensity is needed. W-band ESR measurements revealed that the ...
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Although dynamic nuclear polarization (DNP) has dramatically enhanced solid-state NMR spectral sensitivities of many synthetic materials and some biological macromolecules, recent studies of membrane-protein DNP using exogenously doped paramagnetic radicals as polarizing agents have reported varied and sometimes surprisingly limited enhancement factors. This motivated us to carry out a systemat...
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ژورنال
عنوان ژورنال: Journal of Magnetic Resonance
سال: 2011
ISSN: 1090-7807
DOI: 10.1016/j.jmr.2011.04.001